Labeled sandwich-type electrochemical immunosensor based on Ti3C2Tx/AuNP and Ti3C2Tx/HKUST-1/TB composites for early liver cancer detection.
Mikrochim Acta
; 191(9): 565, 2024 Aug 27.
Article
em En
| MEDLINE
| ID: mdl-39192061
ABSTRACT
A novel sandwich-type electrochemical immunosensor for the detection of the liver cancer marker alpha-fetoprotein (AFP) in human serum is proposed. The two-dimensional MXene material Ti3C2Tx was first prepared using etching and ultrasonic stripping, and then Ti3C2Tx was used to reduce chloroauric acid to form Ti3C2Tx/AuNP composites which were modified on the surface of the glassy carbon electrodes to form probe-type sensors. The Ti3C2Tx/AuNPs provide a large number of binding sites for the AFP capture antibody (Ab1) and increase the electrochemical reaction active site. The Ti3C2Tx/copper metal-organic frameworks HKUST-1 composite was also prepared by solvothermal method and combined with toluidine blue (TB) and AFP detection antibody (Ab2) to form a labeled sandwich-type electrochemical immunosensor. The sensor achieved trace detection of AFP from 0.1 to 100 ng/mL with a detection limit of 0.073 pg/mL and possesses good selectivity, stability, and reproducibility. The sensor performs well in clinical samples and has good potential for clinical applications.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Titânio
/
Técnicas Biossensoriais
/
Alfa-Fetoproteínas
/
Nanopartículas Metálicas
/
Anticorpos Imobilizados
/
Técnicas Eletroquímicas
/
Limite de Detecção
/
Estruturas Metalorgânicas
/
Ouro
/
Neoplasias Hepáticas
Limite:
Humans
Idioma:
En
Revista:
Mikrochim Acta
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
AT
/
AUSTRIA
/
ÁUSTRIA