Tracing Acinetobacter baumannii's Journey from Hospitals to Aquatic Ecosystems.
Microorganisms
; 12(8)2024 Aug 18.
Article
em En
| MEDLINE
| ID: mdl-39203545
ABSTRACT
BACKGROUND:
This study provides a comprehensive analysis of Acinetobacter baumannii in aquatic environments and fish microbiota by integrating culture-dependent methods, 16S metagenomics, and antibiotic resistance profiling.METHODS:
A total of 83 A. baumannii isolates were recovered using culture-dependent methods from intra-hospital infections (IHI) and wastewater (WW) and surface water (SW) samples from two southern Romanian cities in August 2022. The antibiotic susceptibility was screened using disc diffusion, microdilution, PCR, and Whole Genome Sequencing assays.RESULTS:
The highest microbial load in the analyzed samples was found in Glina, Bucharest, for both WW and SW samples across all investigated phenotypes. For Bucharest isolates, the resistance levels corresponded to fluoroquinolones > aminoglycosides > ß-lactam antibiotics. In contrast, A. baumannii from upstream SW samples in TârgoviÈte showed the highest resistance to aminoglycosides. The blaOXA-23 gene was frequently detected in IHI, WW, and SW isolates in Bucharest, but was absent in TârgoviÈte. Molecular phylogeny revealed the presence of ST10 in TârgoviÈte isolates and ST2 in Bucharest isolates, while other minor STs were not specifically correlated with a sampling point. Using 16S rRNA sequencing, significant differences in microbial populations between the two locations was identified. The low abundance of Alphaproteobacteria and Actinobacteria in both locations suggests environmental pressures or contamination events.CONCLUSIONS:
These findings indicate significant fecal contamination and potential public health risks, emphasizing the need for improved water quality monitoring and management.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Microorganisms
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Romênia
País de publicação:
Suíça