Screening and affinity optimization of single domain antibody targeting the SARS-CoV-2 nucleocapsid protein.
PeerJ
; 12: e17846, 2024.
Article
em En
| MEDLINE
| ID: mdl-39224822
ABSTRACT
The coronavirus disease 2019 (COVID-19) pandemic, which caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), lead to a crisis with devastating disasters to global public economy and health. Several studies suggest that the SARS-CoV-2 nucleocapsid protein (N protein) is one of uppermost structural constituents of SARS-CoV-2 and is relatively conserved which could become a specific diagnostic marker. In this study, eight single domain antibodies recognized the N protein specifically which were named pN01-pN08 were screened using human phage display library. According to multiple sequence alignment and molecular docking analyses, the interaction mechanism between antibody and N protein was predicted. ELISA results indicated pN01-pN08 with high affinity to protein N. To improve their efficacy, two fusion proteins were prepared and their affinity was tested. These finding showed that fusion proteins had higher affinity than single domain antibodies and will be used as diagnosis for the pandemic of SARS-CoV-2.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anticorpos de Domínio Único
/
Simulação de Acoplamento Molecular
/
Proteínas do Nucleocapsídeo de Coronavírus
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SARS-CoV-2
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COVID-19
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Anticorpos Antivirais
Limite:
Humans
Idioma:
En
Revista:
PeerJ
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
Estados Unidos