Platinum-based fluorescent nanozyme-driven "loong frolic pearls" multifunctional nanoplatform for tri-mode ultrasensitive detection and synergistic sterilization of Burkholderia gladioli.

ABSTRACT

Rapid and accurate detection of Burkholderia gladioli (B. gladioli) and effective sterilization are crucial for ensuring food safety. Hence, a novel "loong frolic pearls" platform based on platinum-based fluorescent nanozymes (Pt-OCDs) and strand exchange amplification (SEA) was reported. Magnetic nanoparticles were modified on primer SEAF, while Pt-OCDs were covalently coupled with primer SEA-R. The highly efficient amplification capability of SEA permitted the accumulation of a large number of double-labeled amplicons. After magnetic adsorption, the supernatant was detected in reverse direction to collect colorimetric-fluorescence-photothermal signal values, enabling ultra-precise detection within 1 h. Furthermore, the Pt-based multifunctional nanoplatform generated abundant •OH and 1O2, which synergistically attacked B. gladioli and its biofilm, resulting in significant bactericidal efficacy within 30 min. This "triple-detection and double-sterilization" platform has been successfully applied in the field of food analysis with good recovery rates and immediate control over B. gladioli, thus demonstrating promising prospects for broad applications.