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Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube.
Crawford, Ashleigh J; Forjaz, André; Bons, Joanna; Bhorkar, Isha; Roy, Triya; Schell, David; Queiroga, Vasco; Ren, Kehan; Kramer, Donald; Huang, Wilson; Russo, Gabriella C; Lee, Meng-Horng; Wu, Pei-Hsun; Shih, Ie-Ming; Wang, Tian-Li; Atkinson, Mark A; Schilling, Birgit; Kiemen, Ashley L; Wirtz, Denis.
Afiliação
  • Crawford AJ; Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Forjaz A; Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Bons J; Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Bhorkar I; Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Roy T; Buck Institute for Research on Aging, Novato, CA 94945, USA.
  • Schell D; Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Queiroga V; Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Ren K; Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Kramer D; Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Huang W; Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Russo GC; Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Lee MH; Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Wu PH; Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Shih IM; Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Wang TL; Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Atkinson MA; Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Schilling B; Department of Biotechnology, Johns Hopkins Advanced Academic Programs, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Kiemen AL; Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
  • Wirtz D; Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Sci Adv ; 10(39): eadp6285, 2024 Sep 27.
Article em En | MEDLINE | ID: mdl-39331707
ABSTRACT
The fallopian tubes play key roles in processes from pregnancy to ovarian cancer where three-dimensional (3D) cellular and extracellular interactions are important to their pathophysiology. Here, we develop a 3D multicompartment assembloid model of the fallopian tube that molecularly, functionally, and architecturally resembles the organ. Global label-free proteomics, innovative assays capturing physiological functions of the fallopian tube (i.e., oocyte transport), and whole-organ single-cell resolution mapping are used to validate these assembloids through a multifaceted platform with direct comparisons to fallopian tube tissue. These techniques converge at a unique combination of assembloid parameters with the highest similarity to the reference fallopian tube. This work establishes (i) an optimized model of the human fallopian tubes for in vitro studies of their pathophysiology and (ii) an iterative platform for customized 3D in vitro models of human organs that are molecularly, functionally, and microanatomically accurate by combining tunable assembloid and tissue mapping methods.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tubas Uterinas Limite: Female / Humans Idioma: En Revista: Sci Adv Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tubas Uterinas Limite: Female / Humans Idioma: En Revista: Sci Adv Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos