Quantitative changes in the lysosomal vacuolar system of rat hepatocytes during short-term starvation. A morphometric analysis with special reference to macro- and microautophagy.
Cell Tissue Res
; 243(3): 641-8, 1986.
Article
em En
| MEDLINE
| ID: mdl-3955637
ABSTRACT
Ultrastructural morphometric analysis was used to study time-dependent variations in macro- and microautophagy in rat hepatocytes. Except during periods of short-term starvation for up to 24 h, animals were kept under standardized conditions of food intake. In hepatocytes of meal-fed rats the volume fraction of macroautophagic vacuoles is significantly higher at 2300 h, i.e., immediately before food intake, compared to 1100 h, i.e., 12 h following feeding. During fasting, macroautophagy drops to a low level. Microautophagic vacuoles in hepatocytes of meal-fed rats, sacrificed at 1100 or 2300 h respectively, do not show any significant quantitative differences. However, during 12 h of starvation, the volume fraction of microautophagic vacuoles rises significantly, whereas the numerical density remains constant. Subsequently, during the second 12-h period of fasting, the volume fraction of microautophagic vacuoles remains unchanged, but the numerical density increases. Over a period of 24 h of starvation the volume fraction of the total lysosomal system does not change significantly, whereas the numerical density rises. The time-dependent changes of the macroautophagic vacuolar system correlate with the circadian, food-related variations in the protein content of individual hepatocytes from meal-fed animals. The increase in volume fraction and thereafter in number of microautophagic vacuoles, as observed during starvation, coincides with a large decrease in protein content of individual hepatocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fagocitose
/
Autofagia
/
Inanição
/
Vacúolos
/
Organoides
/
Fígado
/
Lisossomos
Limite:
Animals
Idioma:
En
Revista:
Cell Tissue Res
Ano de publicação:
1986
Tipo de documento:
Article