Reliability of exoantigens for differentiating Blastomyces dermatitidis and Histoplasma capsulatum from Chrysosporium and geomyces species.
Diagn Microbiol Infect Dis
; 4(3): 215-21, 1986 Mar.
Article
em En
| MEDLINE
| ID: mdl-3956141
ABSTRACT
A recent study suggested that Chrysosporium species have the same diagnostic antigens as Histoplasma capsulatum and Blastomyces dermatitidis and, thus, compromise the antigenic identification of these pathogens. In light of these findings, studies were undertaken to determine the reliability of the exoantigen tests for identifying B. dermatitidis and H. capsulatum organisms from cultures. Sixty-three slant or shake culture extracts, or both, were derived from C. asperatum, C. keratinophilum, C. parvum, C. pruinosum, C. parvum var. crescens, Geomyces (Chrysosporium) pannorus, B. dermatitidis, and H. capsulatum. These were analyzed by use of a commercial exoantigen kit and exoantigen test reagents obtained from a commercial source. The results of these analyses were compared with those obtained with Centers for Disease Control reagents. Many of the extracts derived from nonpathogenic fungi produced nonspecific precipitin bands when reacted with the kit and reference antisera, particularly the B. dermatitidis antisera. None, however, produced antigens identical to the specific B. dermatitidis A and H. capsulatum H and M antigens. Our findings indicate that the properly controlled immunoidentification procedure is 100% specific for B. dermatitidis and H. capsulatum, and that cross-reacting antigens derived from morphologically similar saparophytic fungi do not pose identification problems.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Blastomyces
/
Chrysosporium
/
Fungos Mitospóricos
/
Histoplasma
/
Antígenos de Fungos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Ano de publicação:
1986
Tipo de documento:
Article