Variations in ADP-ribosylation of nuclear scaffold proteins during the HeLa cell cycle.
Biochem Biophys Res Commun
; 126(2): 840-7, 1985 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-3977890
ABSTRACT
Cell cycle variations in ADP-ribosylation of nuclear scaffold proteins were determined. Nuclei of synchronized cells were isolated and labeled with [32P]NAD before nuclear scaffolds were obtained by digestion of DNA with DNase I and extraction of proteins with 2M NaCl. Autoradiograms revealed the three groups of "lamins" and a species identified as poly (ADP-ribose) polymerase to be the primary ADP-ribosylated proteins. The patterns of modification of nuclear scaffold proteins displayed similar features through the cell cycle. Radioactivity in the lamins increased from 20% in early-S phase to 40% in G1 phase of the next cell cycle.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatos de Dinucleosídeos
/
Núcleo Celular
/
Adenosina Difosfato Ribose
/
Nucleoproteínas
/
Açúcares de Nucleosídeo Difosfato
Limite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1985
Tipo de documento:
Article