Heterogeneous distribution of enzymes among plasma-membrane fragments sedimenting with the microsomal fraction of rat liver.
Biochem J
; 142(3): 667-71, 1974 Sep.
Article
em En
| MEDLINE
| ID: mdl-4377214
ABSTRACT
Plasma-membrane fragments recovered in the microsomal fraction of rat liver homogenates were shown to be heterogeneous in density. It was demonstrated that 5'-nucleotidase, the most commonly used plasma-membrane marker, is concentrated in the lightest subfraction. Two of the published procedures for the isolation of plasma-membrane fragments from the microsomal fraction (Touster et al., 1970; Hinton et al., 1971) are shown to give products which are not representative of all the plasma-membrane fragments of microsomal size, and it is argued that a third procedure (House & Weidemann, 1970) is likely to give a similar product.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microssomos Hepáticos
/
Membrana Celular
/
Fígado
/
Nucleotidases
Limite:
Animals
Idioma:
En
Revista:
Biochem J
Ano de publicação:
1974
Tipo de documento:
Article