Alterations of transcription during heat shock of Saccharomyces cerevisiae.
J Biol Chem
; 257(14): 8405-11, 1982 Jul 25.
Article
em En
| MEDLINE
| ID: mdl-6177697
ABSTRACT
The pattern of polyadenylated RNA of the yeast Saccharomyces cerevisiae changes dramatically upon heat shock. By in vitro translation, we have demonstrated that a 2.9-kilobase heat shock RNA encodes the Mr = 90,000 yeast heat shock protein. Heat shock-responsive genes have been isolated by differential plaque filter hybridization of a recombinant library of yeast DNA inserted in the vector lambda Charon 4. The putative yeast gene products of a number of these recombinants molecules has been determined by in vitro translation of hybrid-selected RNA. We have used one of these hybrid phages (lambda Yhsil) to demonstrate that the heat shock-induced alteration in the level of the 2.9-kilobase polyadenylated RNA which encodes the Mr = 90,000 yeast heat shock protein is regulated at the level of transcription.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poli A
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Saccharomyces cerevisiae
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Transcrição Gênica
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RNA
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Proteínas
Idioma:
En
Revista:
J Biol Chem
Ano de publicação:
1982
Tipo de documento:
Article
País de publicação:
EEUU
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ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
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EUA
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UNITED STATES
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UNITED STATES OF AMERICA
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US
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USA