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Automated assay of alpha-amylase inhibitor proteins by continuous flow analysis.
Anal Biochem ; 130(2): 506-13, 1983 Apr 15.
Article em En | MEDLINE | ID: mdl-6191596
ABSTRACT
A procedure for the automated assay of purified amylase inhibitors was developed. Samples were analyzed at a rate of 60/h using ferricyanide reagent to monitor the suppression of the release of reducing groups from a solution of starch by a calibrated alpha-amylase reagent. In addition to the sequential analysis of individual samples, the use of gradients permitted the continuous analysis of the effect of substrate and of inhibitor concentration. Also described are some of the effects of starch and inhibitor concentration and time of preincubation on the amylase-inhibitor reaction. The procedure was also suitable for the assay of samples of amylase and should be applicable to the determination of the effects of inhibitors on other enzymes which release reducing sugars.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Alfa-Amilases / Amilases Limite: Animals Idioma: En Revista: Anal Biochem Ano de publicação: 1983 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Alfa-Amilases / Amilases Limite: Animals Idioma: En Revista: Anal Biochem Ano de publicação: 1983 Tipo de documento: Article