Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes.
FEBS Lett
; 165(2): 211-5, 1984 Jan 09.
Article
em En
| MEDLINE
| ID: mdl-6198210
ABSTRACT
The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with 125I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with 125I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90% for chromatin.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Proteínas Cromossômicas não Histona
/
Interfase
/
Metáfase
/
Antígenos
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1984
Tipo de documento:
Article