alpha-Melanocyte-stimulating hormone binding and biological activity in a human melanoma cell line.
Cancer Res
; 41(4): 1539-44, 1981 Apr.
Article
em En
| MEDLINE
| ID: mdl-6260342
ABSTRACT
Synthetic alpha-melanocyte-stimulating hormone (alpha-MSH) was found to bind to the plasma membrane of the HM6A human melanoma cell line, using an immunocytochemical method. When treated with 10(-7) to 10(-9) M alpha-MSH, melanoma cells exhibited an increase of intracellular cyclic adenosine 3'5'-monophosphate, followed by stimulation of tyrosinase activity. Significant inhibition of DNA synthesis measured by [3H]thymidine uptake and inhibition of cell growth was found. A retrovirus expression was detected in the supernatant of HM6A cells as assayed by the KC cell syncytium-forming test. In he presence of 10(-7) M alpha-MSH, the number of syncytium-forming units was increased 15-fold. These results demonstrate that alpha-MSH modulates human melanoma differentiation and virus expression in vitro.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hormônios Estimuladores de Melanócitos
/
Melanoma
Limite:
Humans
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1981
Tipo de documento:
Article