Chemical conversion of cytidine residues into 4-thiouridines in yeast tRNAPhe. Determination of the modified cytidines.
Nucleic Acids Res
; 12(11): 4445-53, 1984 Jun 11.
Article
em En
| MEDLINE
| ID: mdl-6377234
ABSTRACT
Treatment of yeast phenylalanine tRNA with pressurized hydrogen sulfide results in conversion of cytidine residues into 4-thiouridine residues. Under conditions leading to an average modification of one cytidine per tRNA molecule 9 positions are thiolated. The 4-thiouridine residues are distributed along the tRNA molecule. Four of the reactive cytidines are located in single-stranded regions Cm32 , C60 , C74 and C75 . The five others are located in base pairs C2, C27, C56 , C61 and C63 . Importance of replacement of an amino group by a thiol group on hydrogen bonding and on biological activity of the modified tRNA is discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Tiouridina
/
Aminoacil-RNA de Transferência
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Citidina
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
1984
Tipo de documento:
Article