A computer-based data analysis system for enzyme-linked immunosorbent assays.
J Immunol Methods
; 65(1-2): 83-95, 1983 Dec 16.
Article
em En
| MEDLINE
| ID: mdl-6418819
ABSTRACT
A computerized system is presented for automating the data collection, processing, and displaying tasks involved in enzyme-linked immunosorbent assays. This system uses a through-the-well absorbance reader of microtiter plates interfaced to a minicomputer running the UNIX operating system. Optical density in each well of a 96-well microtiter plate is recorded as a function of time for up to 10 time points. These data are automatically transmitted to the remote computer. The rate of product formation is then calculated for each well, and a battery of analysis, display, and comparison programs can then be used by the researcher for data presentation. Using the initial rate of reaction as the basis for quantifying enzyme-linked immunosorbent assays focuses on the catalytic property of the enzyme and allows a large dynamic range of the assay on any plate. These programs can be adapted to virtually any mini- or microcomputer with a graphics display or a plotting device. Assuming moderately powerful computing hardware, throughputs of 50 plates per day are easily achieved. The programs work equally well with peroxidase, beta-galactosidase, or alkaline phosphatase conjugated second antibodies, and with whole cell or soluble antigens.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
Computadores
/
Ensaio de Imunoadsorção Enzimática
/
Minicomputadores
/
Técnicas Imunoenzimáticas
Limite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1983
Tipo de documento:
Article