Fluorescence polarization immunoassay of estradiol.
Diagn Immunol
; 1(4): 310-4, 1983.
Article
em En
| MEDLINE
| ID: mdl-6437726
ABSTRACT
Fluorescence polarization immunoassay (FPIA) offers a good alternative to isotopic methods for the determination of drug and hormone levels in biological fluids. We applied it to estradiol, using a fluoresceinated derivative of estradiol and the IgG fraction of a highly specific rabbit antiserum. After determining the optimal operational conditions, FPIA was used for measuring urinary concentrations of free estradiol in normal and pregnant women, after a purification step on a reversed phase cartridge. The method turned out to be rapid (1 min to reach the equilibrium state) and accurate.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Estradiol
Limite:
Animals
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Diagn Immunol
Ano de publicação:
1983
Tipo de documento:
Article