Iron-binding fragments from the N-terminal and C-terminal regions of human lactoferrin.
Biochem J
; 171(2): 321-7, 1978 May 01.
Article
em En
| MEDLINE
| ID: mdl-656048
ABSTRACT
Digestion of lactoferrin with pepsin at pH3.0 gave an iron-binding half-molecule that represents the C-terminal part of the native protein. Tryptic or chymotryptic digestion of 30%-iron-saturated lactoferrin yielded the N- and C-terminal half molecules, which could be separated by DEAE-Sephadex chromatography. The N- and C-terminal fragments did not show any immunological cross-reaction. The carbohydrate of lactoferrin was distributed equally between the two fragments.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ferro
/
Lactoferrina
/
Lactoglobulinas
Limite:
Humans
Idioma:
En
Revista:
Biochem J
Ano de publicação:
1978
Tipo de documento:
Article