Isolation and identification of testosterone from the serum and testes of the American lobster (Homarus americanus).
Gen Comp Endocrinol
; 54(3): 429-32, 1984 Jun.
Article
em En
| MEDLINE
| ID: mdl-6735160
ABSTRACT
Testosterone was isolated and quantified from male lobster serum and testes by solvent extraction, sequential thin-layer chromatography, and high-performance liquid chromatography. The identity of the isolated steroid was established by its isopolarity and isomorphicity with authentic radiolabeled testosterone and its acetate derivative. The concentrations of testosterone were determined by high-performance liquid chromatography, by a double-isotope derivative assay, and by radioimmunoassay. The concentrations of testosterone as determined by the three methods were the same and were 0.3 ng/ml and 14.3 ng/g in lobster serum and testes, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testículo
/
Testosterona
/
Nephropidae
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Gen Comp Endocrinol
Ano de publicação:
1984
Tipo de documento:
Article