Effect of iron concentration in the growth medium on the sensitivity of Pseudomonas aeruginosa to pyocin S2.
J Biochem
; 87(1): 323-31, 1980 Jan.
Article
em En
| MEDLINE
| ID: mdl-6766926
ABSTRACT
The iron concentration in the growth medium was found to affect the susceptibility of Pseudomonas aeruginosa PML1550 to pyocin S2, a bacteriocin. The efficiency of killing by pyocin S2 was very low when the indicator cells were grown in an iron-rich medium. The capacity of these cells to adsorb pyocin S2 was reduced. Cultivation under limitation of iron (1 microM or less) was necessary to produce a fully sensitive cell population. The growth under iron limitation was accompanied by the appearance of four protein components in the outer membrane of the cells. Nine mutants resistant to pyocin S2 were isolated and their outer membranes were analyzed. They all lacked one component (Fe-b protein) as well as the adsorption capacity for pyocin S2. These findings suggest a possible role of this protein as the receptor for pyocin S2.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
/
Piocinas
/
Bacteriocinas
/
Ferro
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Biochem
Ano de publicação:
1980
Tipo de documento:
Article