Assay of etozolin and its main metabolite, ozolinone, in plasma by high performance liquid chromatography.
Arzneimittelforschung
; 30(10): 1788-90, 1980.
Article
em En
| MEDLINE
| ID: mdl-7192115
ABSTRACT
A sensitive and specific method for the determination of the diuretic Ethyl (Z)-(3-methyl-4-oxo-5-piperidino-thiazolidin-2-ylidene) acetate (etozolin, Elkapin) and its pharmacologically active main metabolite, (Z)-(3-methyl-4-oxo-5-piperidino-thiazolidin-2-ylidene) acetic acid (ozolinone), at therapeutic concentrations in plasma is described. The method is based on high performance liquid chromatography and the use of two structurally similar internal standards. Etozolin and its metabolite are extracted from the plasma into dichloromethane at pH 9 and pH 5, respectively, and the resulting residues are analyzed on a silica gel column. The elution peaks are detected by UV absorption at 281 nm. The sensitivity for both compounds is 20 ng/ml plasma. The precision of the method is about +/- 5%. The applicability to pharmacokinetic studies of etozolin is shown.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tiazóis
/
Benzotiadiazinas
/
Inibidores de Simportadores de Cloreto de Sódio
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
Arzneimittelforschung
Ano de publicação:
1980
Tipo de documento:
Article
País de publicação:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY