Phenobarbital specific antibody production: preparation of 5-phenyl-5-(4-aminobutyl) barbituric acid-bovine serum albumin conjugate.

The aminobutyl derivative of phenobarbital, 5-phenyl-5-(4-aminobutyl)barbituric acid hydrochloride, was synthesized through two synthetic pathways for the preparation of immunogen in production of phenobarbital specific antibody. The produced antiserum had high titer, specificity, affinity and sensitivity (0.5ng/ml), when examined by radioimmunoassay.