Rapid detection of amphetamine in urine by micro thin-layer chromatography and fluorescence.
Clin Toxicol
; 13(5): 545-9, 1978 Dec.
Article
em En
| MEDLINE
| ID: mdl-750161
ABSTRACT
A rapid, inexpensive, and simple screening procedure for the detection of amphetamine abuse was developed for use by laboratories without sophisticated equipment. A small volume of extract from a pH-adjusted urine specimen is used to spot a high-resolution micro TLC plate. The developed TLC plate is sprayed with a solution of fluorescamine in dry acetone. When viewed under ultraviolet illumination, amphetamines and other compounds with a primary amino group complexed with fluorescamine appear as greenish or bluish-white fluorescent spots. Secondary or tertiary amines do not react with fluorescamine. About 20 min is required to perform the procedure; the lower limit of detectability is approximately 100 ng/ml urine.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anfetamina
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Clin Toxicol
Ano de publicação:
1978
Tipo de documento:
Article