Ethacrynic acid inhibitable Ca2+ and Mg2+-activated membrane adenosine triphosphatase in rat mast cells.
Clin Exp Immunol
; 30(1): 160-7, 1977 Oct.
Article
em En
| MEDLINE
| ID: mdl-75076
ABSTRACT
A crude plasma membrane fraction from the homogenate of purified rat mast cells demonstrates a high degree of Ca2+-dependent and Mg2+-dependent adenosine triphosphatase (ATPase) activity. The microsomal and mitochondrial fractions show negligible amounts of the Ca2+ and Mg2+-activated ATPases. The broad ATPase inhibitor, ethacrynic acid, effectively blocks the mast cell ATPase activity while ouabain demonstrates little inhibitory effect. Correspondingly, ethacrynic acid inhibits histamine release from antigen-challenged mast cells while ouabain does not. Both ATPase inhibition and histamine release inhibition by ethacrynic acid require the presence of the olefinic bond in the ethacrynic acid molecule.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cálcio
/
Adenosina Trifosfatases
/
Ácido Etacrínico
/
Magnésio
/
Mastócitos
Limite:
Animals
Idioma:
En
Revista:
Clin Exp Immunol
Ano de publicação:
1977
Tipo de documento:
Article