Alkalinization of acidic cellular compartments following cell swelling.
FEBS Lett
; 338(1): 27-30, 1994 Jan 24.
Article
em En
| MEDLINE
| ID: mdl-7508402
ABSTRACT
Osmotic swelling of rat hepatocytes increases fluorescence of Acridine orange and of fluorescein isothiocyanate (FITC)-dextran, both indicative of alkalinization of acidic intracellular vesicles. Similar to osmotic cell swelling, insulin and glutamine lead to an increase in Acridine orange fluorescence, an effect virtually abolished upon osmotic reversal of glutamine-induced cell swelling. Barium, which blocks K+ channels in the plasma membrane, similarly leads to cell swelling and increase of Acridine orange fluorescence. Since proteolysis is governed by lysosomal pH, these observations indicate that the anti-proteolytic action of osmotic cell swelling is mediated by lysosomal alkalinization. Thereby, insulin, glutamine and barium probably exert their anti-proteolytic action by cell swelling and subsequent lysosomal alkalinization.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compartimento Celular
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Áustria