Evaluation of a new cytotoxicity assay for Clostridium perfringens type D epsilon toxin.
FEMS Microbiol Lett
; 116(2): 161-7, 1994 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-7512061
ABSTRACT
A new cytotoxicity assay for determining the activity of epsilon toxin produced by Clostridium perfringens type D has been developed. Viability of cultured cells was determined by the ability of only live cells to convert 5-(3-carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3-(4- sulfophenyl)tetrazolium to the coloured product formazan in the presence of phenazine methosulfate. Of the 12 cell lines tested, only the MDCK cell line was susceptible to epsilon toxin. Specificity was confirmed by the ability of only specific monoclonal antibodies to inhibit cytotoxicity. Good correlation was obtained with the mouse lethality assay (r = 0.991) and over a wide range of viability (15-75%) as determined by ethidium bromide/acridine orange staining (r = 0.995).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Sobrevivência Celular
/
Clostridium perfringens
Limite:
Animals
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Reino Unido