Brevetoxin PbTx-2 immunology: differential epitope recognition by antibodies from two goats.
Toxicon
; 32(8): 883-90, 1994 Aug.
Article
em En
| MEDLINE
| ID: mdl-7527163
ABSTRACT
The epitopic regions of the brevetoxin PbTx-3 molecule, produced by the marine dinoflagellate Ptychodiscus brevis, have been identified by structural modification at three distinct regions of the toxin. These are the A-ring lactone region of the molecule, the K-ring side-chain and the H-ring. The modified PbTx-3 derivatives were tested for their ability to bind brevetoxin goat antisera directed against the PbTx-3 molecule, by radioimmunoassay. The results showed that at least two major epitopes and one minor epitope are recognized the A-ring lactone region of the molecule and the K-ring side-chain, and the H-ring. The results illustrate the variety of antibodies which may be produced, even within a species, and suggests that epitope characterization is important in the development of assays which are to be employed in seafood safety issues.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxocinas
/
Toxinas Marinhas
/
Epitopos
/
Neurotoxinas
Limite:
Animals
Idioma:
En
Revista:
Toxicon
Ano de publicação:
1994
Tipo de documento:
Article