Binding studies of cationic thymidyl deoxyribonucleic guanidine to RNA homopolynucleotides.
Proc Natl Acad Sci U S A
; 92(15): 7051-5, 1995 Jul 18.
Article
em En
| MEDLINE
| ID: mdl-7542784
ABSTRACT
Deoxyribonucleic guanidine is a potential antisense agent that is generated via the replacement of the negative phosphodiester linkages of DNA [--O--(PO2-)--O--] with positively-charged guanidinium (g) linkages [--NH--C(==NH2+)--NH--]. A pentameric thymidyl deoxyribonucleic guanidine molecule [d(Tg)4T-azido] has been shown to base pair specifically to poly(rA) with an unprecedented affinity. Both double and triple strands consisting of one and two equivalents of d(Tg)4T-azido paired with one equivalent of poly(rA) are indicated by thermal denaturation experiments. At an ionic strength of 0.22, the five bases of d(Tg)4T-azido are estimated to dissociate from a double helix with poly(rA) at > 100 degrees C! The effect of ionic strength on thermal denaturation is very pronounced, with stability greatest at low ionic strengths. The method of continuous variation indicates that there is an equilibrium complex with a molar ratio of d(Tg) to r(Ap) or d(Ap) of 21. Based on this evidence, models of the structures of d(Tg)9T-azido bound to r(Ap)9A are proposed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polirribonucleotídeos
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DNA
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RNA
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Elementos Antissenso (Genética)
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Guanidinas
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Ácidos Nucleicos Heteroduplexes
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos