Mutation of a highly conserved base in the yeast mitochondrial 21S rRNA restricts ribosomal frameshifting.
Mol Gen Genet
; 248(2): 207-16, 1995 Jul 28.
Article
em En
| MEDLINE
| ID: mdl-7544431
ABSTRACT
A mutation shown to cause resistance to chloramphenicol in Saccharomyces cerevisiae was mapped to the central loop in domain V of the yeast mitochondrial 21S rRNA. The mutant 21S rRNA has a base pair exchange from U2677 (corresponding to U2504 in Escherichia coli) to C2677, which significantly reduces rightward frameshifting at a UU UUU UCC A site in a +1 U mutant. There is evidence to suggest that this reduction also applies to leftward frameshifting at the same site in a -1 U mutant. The mutation did not increase the rate of misreading of a number of mitochondrial missense, nonsense or frameshift (of both signs) mutations, and did not adversely affect the synthesis of wild-type mitochondrial gene products. It is suggested here that ribosomes bearing either the C2677 mutation or its wild-type allele may behave identically during normal decoding and only differ at sites where a ribosomal stall, by permitting non-standard decoding, differentially affects the normal interaction of tRNAs with the chloramphenicol resistant domain V. Chloramphenicol-resistant mutations mapping at two other sites in domain V are described. These mutations had no effect on frameshifting.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Biossíntese de Proteínas
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RNA Fúngico
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RNA Ribossômico
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Resistência ao Cloranfenicol
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Mutação da Fase de Leitura
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Mitocôndrias
Idioma:
En
Revista:
Mol Gen Genet
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Alemanha