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Dietary lipids and calorie restriction affect mammary tumor incidence and gene expression in mouse mammary tumor virus/v-Ha-ras transgenic mice.
Fernandes, G; Chandrasekar, B; Troyer, D A; Venkatraman, J T; Good, R A.
Afiliação
  • Fernandes G; Departments of Medicine, University of Texas Health Science Center, San Antonio 78284-7874, USA.
Proc Natl Acad Sci U S A ; 92(14): 6494-8, 1995 Jul 03.
Article em En | MEDLINE | ID: mdl-7604020
We have studied the effects of food restriction (FR) and substitution of fish oil (FO; omega 3) for corn oil (CO; omega 6) on breast tumor incidence and survival in mouse mammary tumor virus/v-Ha-ras transgenic (Onco) mice. The diets were as follows: group 1, 5% (wt/wt) CO fed ad libitum (AL); group 2, 5% CO, restricted calories (40% fewer calories than AL; FR); group 3, 20% CO fed AL; and group 4, 20% FO fed AL. After 3 years, 40% of FR Onco (group 2) mice were alive, whereas there were no survivors in the other three groups. Similarly, tumor incidence was reduced to 27% (5 out of 18) in FR animals (group 2), whereas it was 83% (11 out of 13) in group 1 mice, 89% (16 out of 18) in group 3 mice, and 71% (10 out of 14) in group 4 mice. These protective effects of FR on survival and tumor incidence were paralleled by higher expression of the tumor suppressor gene p53 (wild type) and free-radical scavenging enzymes (catalase and superoxide dismutase) in breast tumors. Immunoblotting showed less ras gene product, p21, and increased p53 levels in the tumors of FR mice. In addition, FR decreased RNA levels of c-erbB-2, interleukin 6, and the transgene v-Ha-ras in tumors. In contrast, analysis of hepatic mRNA from tumor-bearing FR mice revealed higher expression of catalase, glutathione peroxidase, and superoxide dismutase. Survival and tumor incidence were not influenced significantly by dietary supplementation with FO in place of CO. Taken together, our studies suggest that moderate restriction of energy intake significantly inhibited the development of mammary tumors and altered expression of cytokines, oncogenes, and free-radical scavenging enzymes.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Gorduras na Dieta / Expressão Gênica / Genes ras / Vírus do Tumor Mamário do Camundongo / Dieta Redutora / Neoplasias Mamárias Experimentais Tipo de estudo: Incidence_studies / Prognostic_studies / Risk_factors_studies Limite: Animals / Female / Humans Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 1995 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Gorduras na Dieta / Expressão Gênica / Genes ras / Vírus do Tumor Mamário do Camundongo / Dieta Redutora / Neoplasias Mamárias Experimentais Tipo de estudo: Incidence_studies / Prognostic_studies / Risk_factors_studies Limite: Animals / Female / Humans Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 1995 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos