Chromosome microdissection identifies cryptic sites of DNA sequence amplification in human ovarian carcinoma.
Cancer Res
; 55(15): 3380-5, 1995 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-7614475
ABSTRACT
DNA sequence amplification contributes to the multistep process of carcinogenesis, and overexpression of amplified genes has been shown to contribute to the malignant phenotype. Cytogenetic analyses of human tumor cells, including ovarian malignancies, frequently show cytological evidence of DNA amplification in the form of double minutes and homogeneously staining regions. In this report, we have combined the techniques of chromosome microdissection and fluorescence in situ hybridization (P. S. Meltzer et al., Nat. Genet., 1 24-28, 1992) to identify the composition and chromosomal origin of seven homogeneously staining regions from seven cases of ovarian cancer. Twelve specific chromosome band regions were identified as amplified including 11q, 12p, 16p, 19p, and 19q. These results provide important insights into the organization of amplified sequences within ovarian malignancies and add further to our recognition of regions likely to harbor genes important to the development or progression of ovarian cancer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ovarianas
/
Hibridização in Situ Fluorescente
/
Técnicas de Amplificação de Ácido Nucleico
Limite:
Aged
/
Female
/
Humans
/
Middle aged
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos