Role of protein synthesis in interleukin 1 alpha-induced prostaglandin production in ovine astroglia.
Prostaglandins Leukot Essent Fatty Acids
; 53(1): 69-72, 1995 Jul.
Article
em En
| MEDLINE
| ID: mdl-7675826
ABSTRACT
Experiments were done to determine whether interleukin 1 alpha (IL-1 alpha) induces prostaglandin (PG) synthesis in cultured astroglia and to examine the role of enhanced cyclooxygenase 2 (COX 2) synthesis in these responses. Immunologically identified fetal ovine astrocytes were exposed to IL-1 alpha or to media alone. Application of 10 ng/ml IL-1 alpha increased the production of PGF2 alpha for 2-24 h. Coincubation of IL-1 alpha with actinomycin D (1 microgram/ml) or cycloheximide (10 micrograms/ml) completely blocked the increase in PGF2 alpha. Immunoblot analyses revealed that the synthesis of COX2 was not increased by IL-1 alpha at 4 h. However, with incubation of IL-1 alpha for 8-12 h, COX2 levels were increased. These results indicate that longer periods than 4 h are necessary to increase COX2 protein, suggesting that proteins other than COX2 are involved in early IL-1 alpha-induced PG synthesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prostaglandinas
/
Astrócitos
/
Interleucina-1
/
Proteínas do Tecido Nervoso
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Prostaglandins Leukot Essent Fatty Acids
Assunto da revista:
ENDOCRINOLOGIA
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos
País de publicação:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM