Activated neutrophils and neutrophil activators in human milk: increased expression of CD11b and decreased expression of L-selectin.
J Leukoc Biol
; 54(2): 97-104, 1993 Aug.
Article
em En
| MEDLINE
| ID: mdl-7689631
ABSTRACT
Human milk neutrophils and macrophages were examined by flow cytometry to determine whether they displayed phenotypic markers of activation. The markers were CD11b and L-selectin, which are increased or shed, respectively, from the surface of activated neutrophils. Phenotypic features of milk neutrophils and macrophages were similar to blood neutrophils stimulated with fMLP plasma membrane expression of CD11b was increased and L-selectin was decreased. After blood neutrophils were incubated in acellular milk, their expression of CD11b increased and L-selectin decreased. The activation was not affected by trypsin but was significantly decreased by treating acellular milk with chloroform or ether. Sedimentation studies suggested that particulate fractions of milk were active. Further, the activation was partly blocked by treating target blood neutrophils with cytochalasin B. Thus, human milk neutrophils are activated, and the activation may be due partly to phagocytosis of membranous structures in milk.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Moléculas de Adesão Celular
/
Antígeno de Macrófago 1
/
Leite Humano
/
Neutrófilos
Limite:
Humans
Idioma:
En
Revista:
J Leukoc Biol
Ano de publicação:
1993
Tipo de documento:
Article