Rapid synchrony of nuclear and cytosolic Ca2+ signals activated by muscarinic stimulation in the human tumour line TE671/RD.
Cell Calcium
; 16(6): 491-9, 1994 Dec.
Article
em En
| MEDLINE
| ID: mdl-7712542
ABSTRACT
The functional properties of muscarinic cholinergic receptors have been studied in single cells of the TE671/RD human line. Muscarinic stimulation causes large and quick elevations of cytosolic Ca2+ in the majority of the cells; these persist even in the absence of external Ca2+. Electrophysiological experiments reveal, in addition to the expected nicotinic current, the activation of a K(+)-specific current in response to muscarine. The cell nucleus appears freely permeable to the acid form of Fura-2 and the cytosolic Ca2+ changes easily spread into the nucleus, suggesting free diffusion through nuclear pores. Under appropriate Fura-2 loading conditions, fast (up to 0.5 Hz) Ca2+ oscillations can be observed, usually originating from a restricted cytosolic region. This phenomenon is reflected in fast oscillations of the Ca(2+)-activated K+ current.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Núcleo Celular
/
Cálcio
/
Receptores Muscarínicos
/
Citosol
/
Muscarina
Limite:
Humans
Idioma:
En
Revista:
Cell Calcium
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Itália