[Effects of 17 beta-estradiol and growth factors on cell proliferation and c-fos gene expression, in vitro, in endometrial cells in normal growth]. / Effets de l'estradiol-17 beta et de facteurs de croissance sur la prolifération cellulaire et l'expression du gène c-fos, in vitro, dans les cellules endométriales en croissance normale.
C R Seances Soc Biol Fil
; 188(4): 387-96, 1994.
Article
em Fr
| MEDLINE
| ID: mdl-7736261
ABSTRACT
Glandular epithelial cells (GEC) and stromal cells (SC) were isolated from guinea-pig endometrium and cultured separately. After the cells were made quiescent by serum depletion, cell proliferation and c-fos gene expression were investigated. Estradiol-17 beta (E2) alone had no effect on cell proliferation or c-fos gene expression. Cell proliferation was observed in SC and GEC after stimulation with insulin plus EGF and a supplementary effect was obtained when E2 was associated with these factors only in GEC. In GEC, insulin plus EGF had no effect on c-fos expression but an effect was observed when E2 was associated with these factors or with a protein synthesis inhibitor, cycloheximide (Chx) or puromycine. An E2 effect in the presence of Chx was also observed in SC. These data suggest the presence of a labile protein preventing the in vitro estrogenic action in endometrial cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator de Crescimento Insulin-Like I
/
Expressão Gênica
/
Genes fos
/
Endométrio
/
Fator de Crescimento Epidérmico
/
Estradiol
Limite:
Animals
Idioma:
Fr
Revista:
C R Seances Soc Biol Fil
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
França