Differential expression of the alpha- and beta-isoforms of protein kinase C in peripheral blood T and B cells from young and elderly adults.
Mech Ageing Dev
; 77(3): 197-211, 1995 Jan 13.
Article
em En
| MEDLINE
| ID: mdl-7739267
ABSTRACT
The expression of alpha- and beta-isoforms of protein kinase C (PKC) was analyzed in the peripheral blood T and B cells from 11 elderly and young humans. Immunoblot analysis with isoenzyme specific antibodies showed that T cells from five of 11 elderly subjects exhibited selective reductions in PKC alpha which was < 60% of those in young subjects whereas the levels of PKC beta were comparable to T cells of young subjects. No age-related reductions of PKC alpha or beta were observed in B cells. Among individual elderly subjects, the reductions in T cell PKC alpha were not associated with lower levels of PKC beta thereby resulting in only approximately 60-70% reductions of combined PKC alpha plus PKC beta. In addition, the functional properties of PKC in stimulated T cells of elderly subjects with respect to activation/translocation were comparable to T cells of young subjects. These results suggest that selective alterations in PKC isoenzymes can occur in human T cells during aging which may not be readily apparent in standard enzymatic assays and may contribute to aberrancies in intracellular signal transduction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Sanguíneas
/
Proteína Quinase C
/
Envelhecimento
/
Linfócitos B
/
Linfócitos T
/
Isoenzimas
Limite:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Mech Ageing Dev
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos