Isolation and characterization of sheep lactoferrin, an inhibitor of platelet aggregation and comparison with human lactoferrin.
Biochim Biophys Acta
; 1243(1): 25-32, 1995 Jan 18.
Article
em En
| MEDLINE
| ID: mdl-7827104
ABSTRACT
Highly purified sheep lactoferrin was isolated from ovine whey in a single chromatographic step (FPLC) it was characterized by electrophoresis, N-terminal sequence determination and compared with lactoferrins from other species. Sheep and human lactoferrins inhibited thrombin-induced platelet aggregation (median inhibitory concentration IC50 5 and 4 microM, respectively). Pepsin hydrolysates of human and sheep lactoferrins were fractionated by reverse-phase high-performance liquid chromatography and only one peak was an inhibitor of platelet aggregation. The sheep or human lactoferrin binding to platelets was studied.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inibidores da Agregação Plaquetária
/
Agregação Plaquetária
/
Lactoferrina
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
França