Affinity retardation chromatography: characterization of the method and its application. The description of low affinity laminin self-interactions.
Anal Biochem
; 222(1): 140-8, 1994 Oct.
Article
em En
| MEDLINE
| ID: mdl-7856840
ABSTRACT
Affinity retardation chromatography (ARC), a method for the examination of low-affinity interactions, is mathematically described in order to characterize the method itself and to estimate binding coefficients of self-assembly domains of basement membrane protein laminin. Affinity retardation was determined by comparing the elutions on a "binding" and on a "nonreacting" column. It depends on the binding coefficient, the concentrations of both ligands, and the nonbinding elution position. Half maximal binding of the NH2-terminal domain of laminin B1-short arm to the A- and/or B2-short arms was estimated to occur at 10-17 microM for noncooperative and at < or = 3 microM for cooperative binding. A model of the laminin polymerization, postulating two levels of cooperative binding behavior, is described.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia de Afinidade
/
Laminina
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Suíça
País de publicação:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
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US
/
USA