Ultrastructure of the 5-hydroxytryptamine3 receptor.
J Neurochem
; 64(3): 1401-5, 1995 Mar.
Article
em En
| MEDLINE
| ID: mdl-7861173
ABSTRACT
We have determined the ultrastructure of 5-hydroxytryptamine3 (5-HT3) serotonin receptors purified from NG108-15 mouse neuroblastoma x rat glioma cells by electron microscopic examination of receptor particles embedded in uranyl acetate stain and metal replicas of rapidly frozen receptors. The 5-HT3 receptor can be modelled as a cylinder 11 nm in length and 8 nm in diameter with a closed end and a central cavity 3 nm in diameter. Analysis of the rotational symmetry of single receptor particles indicates that the 5-HT3 receptor is composed of five subunits arranged symmetrically around a central cavity. Together with evidence obtained for related proteins in other studies using ultrastructural, biochemical, or electrophysiological methods, our observations suggest that all members of the ligand-gated ion channel superfamily may possess a pentameric quaternary structure.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Serotonina
Limite:
Animals
Idioma:
En
Revista:
J Neurochem
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Reino Unido