Calcium-modulated inward rectification of a calcium-activated potassium channel in neurons.
J Neurophysiol
; 72(6): 3023-5, 1994 Dec.
Article
em En
| MEDLINE
| ID: mdl-7897508
ABSTRACT
1. Inward rectification of a calcium-activated K+ channel in neurons cultured from the hypothalamus and brain stem of 1-day-old rats was studied by using patch-clamp techniques. A big conductance calcium-activated K+ channel with a slow gating rate was observed in inside-out patches. With symmetrical K+ across patches, inward conductance of this calcium-activated K+ channel was 216 +/- 14 (SE) pS (n = 4 patches), which changed little as different [Ca2+] was included in the bath solution. Outward conductance of this calcium-activated K+ channel was regulated by [Ca2+] in the bath solution and was 74 +/- 15 pS with 500 microM Ca2+. The higher level of Ca2+ on the intracellular side of the membrane caused the larger degree of rectification. Mg2+ only had a minor effect on rectification of this calcium-activated K+ channel.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais de Potássio
/
Cálcio
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
J Neurophysiol
Ano de publicação:
1994
Tipo de documento:
Article