CD8+ cytolytic T lymphocytes become infected in vitro in the process of killing HIV-1-infected target cells.
Eur J Immunol
; 24(3): 531-6, 1994 Mar.
Article
em En
| MEDLINE
| ID: mdl-7907291
ABSTRACT
In the present study the requirements for in vitro infection of antigen-specific CD8+ cytotoxic T lymphocytes (CTL) with human immunodeficiency virus -1(HIV-1) were investigated. CD3+CD8+CD4- HIV-1 nef-specific CTL become infected with HIV-1 after short-term co-culture with HLA-matched HIV-1-infected CD20+ B lymphoblastoid cells (B-LCL) which are specifically killed. Similar results were observed with an allospecific CD8+ CTL population. In addition, co-culture experiments showed that once infected with HIV-1, these CD8+ CTL could spread the infection further to uninfected CD4+ lymphocytes. In contrast, CD8+ CTL did not become infected with HIV-1 when co-cultured with HLA-mismatched HIV-1-infected B-LCL which are not killed. These observations in vitro could have relevance in peripheral lymphoid organs contributing to the progressive decrease of HIV-specific CD8+ CTL activity that is associated with the progression to AIDS.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T Citotóxicos
/
Infecções por HIV
/
Subpopulações de Linfócitos T
Limite:
Humans
Idioma:
En
Revista:
Eur J Immunol
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Itália