Species identification of trichostrongyle nematodes by PCR-linked RFLP.
Int J Parasitol
; 24(2): 291-3, 1994 Apr.
Article
em En
| MEDLINE
| ID: mdl-7913087
ABSTRACT
Restriction fragment length polymorphisms (RFLP) of the second internal transcribed spacer (ITS-2) of ribosomal DNA were examined for 6 species of ruminant nematodes; Trichostrongylus colubriformis, T. axei, T. vitrinus, Haemonchus contortus, Teladorsagia circumcincta and Cooperia oncophora. The ITS-2 of each species was amplified by PCR and restricted with the endonucleases DraI, HinfI, RsaI and VspI. Using this approach, the 6 species could be distinguished. No intraspecific variation in RFLP's has been detected so far in the ITS-2 of these species, suggesting that this technique has considerable potential for the identification of eggs of different nematode species in faecal samples.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trichostrongyloidea
/
Tricostrongiloidíase
/
Polimorfismo de Fragmento de Restrição
/
Ruminantes
/
DNA Ribossômico
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Int J Parasitol
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Austrália