A preliminary evaluation of drug biotransformation in hepatocytes of genetically defined rat strains.
Physiol Res
; 43(2): 131-5, 1994.
Article
em En
| MEDLINE
| ID: mdl-7918339
ABSTRACT
This study was directed to use the genetically developed isoprenaline-sensitive (S), isoprenaline-resistant (R) and spontaneous hypertensive rats (SHR) as standard diseased animal models for in vitro liver function evaluation of drug biotransformation. Hepatic hexobarbital hydroxylase and glutathione transferase (GST) were evaluated by using hexobarbital and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates, at concentrations of 0.21 mmol/l and 1 mmol/l, respectively. The assay was conducted by using isolated hepatocytes in suspension and hepatocytes in a bioreactor configuration. The data demonstrate that there are certain cellular pharmacokinetic differences in hexobarbital hydroxylase and GST activities in hepatocytes obtained from Wistar, SHR, R and S strains which can be better demonstrated, when using the model of perfused and immobilized hepatocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glutationa Transferase
/
Oxigenases de Função Mista
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Physiol Res
Assunto da revista:
FISIOLOGIA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
República Tcheca