Identification and characterization of a TNF alpha antagonist derived from a monoclonal antibody.
Mol Immunol
; 31(14): 1059-67, 1994 Oct.
Article
em En
| MEDLINE
| ID: mdl-7935497
ABSTRACT
Peptides derived from the CDRs of the anti-TNF alpha monoclonal antibody Di62 were tested for inhibition of binding of Di62 to TNF alpha as well as of TNF alpha to its 55 and 75 kDa receptor. A peptide derived from the CDR1 of the light chain was shown to specifically inhibit Di62 binding to TNF alpha with markedly higher activity (Ki = 4 microM) than all other CDR-derived peptides. This peptide also significantly inhibited binding of TNF alpha to its 55 and 75 kDa receptor and protected L929 cells from the cytotoxic effect of TNF alpha (IC50 = 6 microM). The C-terminal region of this peptide, which is homologous to the 55 and 75 kDa TNF receptor, was found to be essential for activity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Região Variável de Imunoglobulina
/
Fator de Necrose Tumoral alfa
/
Anticorpos Monoclonais
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mol Immunol
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Alemanha