PCR-RFLP typing of the bovine myoglobin gene.
Anim Genet
; 25(3): 187-9, 1994 Jun.
Article
em En
| MEDLINE
| ID: mdl-7943954
ABSTRACT
We have identified substitutions in the 3' untranslated region of the bovine myoglobin gene, one of which affects an MboII restriction enzyme site resulting in a bi-allelic restriction fragment length polymorphism. Co-dominant inheritance of the alleles in three reference families was observed using a polymerase chain reaction--restriction fragment length polymorphism assay. The distribution of the alleles seems characteristic of cattle type--one of the alleles was not detected in purely taurine breeds. Furthermore, we mapped, using the polymerase chain reaction on a bovine-rodent somatic cell hybrid panel, the myoglobin gene to bovine chromosome five. It is therefore syntenic with gamma-interferon and insulin-like growth factor in which we have not found polymorphism. The myoglobin locus therefore serves as a type one marker on bovine chromosome five.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bovinos
/
Mioglobina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Anim Genet
Assunto da revista:
GENETICA
/
MEDICINA VETERINARIA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Quênia