Characterization of three putative Serpulina hyodysenteriae hemolysins.
Microb Pathog
; 16(4): 269-82, 1994 Apr.
Article
em En
| MEDLINE
| ID: mdl-7968456
Serpulina hyodysenteriae hemolysin is though to be an important virulence factor in swine dysentery. One gene, tlyA, previously called tly, encoding a hemolysin in S. hyodysenteriae strain B204 has been characterized (Muir et al. Infect Immun 1992; 60: 529-35). Two other genes of S. hyodysenteria strain B204, designated tlyB and tlyC, encoding hemolytic proteins in Escherichia coli strain DH5 alpha were cloned and sequenced. The tlyB and tlyC genes, when expressed in E. coli, encode heat-labile, protease-sensitive proteins which exhibit both hemolytic and cytotoxic activity in vitro. The calculated molecular weights of the tlyB and tlyC gene products are 93.3 kDa and 30.8 kDa, respectively. The tlyB gene product has sequence homology with the Clp proteins, whereas for the tlyC-encoded protein no homology with other protein sequences was observed. Southern hybridization showed that the tlyB and tlyC genes are present in a single copy on the chromosome of S. hyodysenteriae.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Brachyspira hyodysenteriae
/
Genes Bacterianos
/
Proteínas Hemolisinas
Limite:
Humans
Idioma:
En
Revista:
Microb Pathog
Assunto da revista:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Holanda
País de publicação:
Reino Unido