Characterization of Ca(2+)-ATPase in rod outer segment disk membranes.
Biochem Biophys Res Commun
; 204(2): 813-9, 1994 Oct 28.
Article
em En
| MEDLINE
| ID: mdl-7980547
ABSTRACT
The Ca(2+)-pump isolated in rod outer segment disk membranes as aspartylphosphate intermediate E-P has been characterized the 100 kDa phosphoprotein was completely inhibited by thapsigargin, was not sensitive to digestion by calpain, and displayed a tryptic digestion pattern with the formation of two autophosphorylatable fragments of about 55 and 35 kDa. These results are typical of the calcium pumps of sarcoplasmic or endoplasmic reticulum calcium and differ from those of plasma membrane, such as the Ca(2+)-ATPase of the red blood cells, here shown as controls. The physiological role of calcium pump in disk membranes of vertebrate photoreceptors is discussed in terms of intracellular calcium buffering.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Segmento Externo da Célula Bastonete
/
ATPases Transportadoras de Cálcio
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Itália