Roles of prostaglandins and intracellular free calcium mobilisation in epidermal growth factor-induced proliferation of human amnion cells.
Br J Obstet Gynaecol
; 101(10): 894-900, 1994 Oct.
Article
em En
| MEDLINE
| ID: mdl-7999692
ABSTRACT
OBJECTIVE:
To investigate the mechanisms which regulate the growth of human amnion cells.DESIGN:
A prospective descriptive study.SUBJECTS:
Women undergoing caesarean section at term before the onset of labour.INTERVENTIONS:
Amnion cells were cultured in monolayer. MAIN OUTCOMEMEASURES:
Cell cycle analysis, intracellular calcium levels, prostaglandin (PG) production rates.RESULTS:
Epidermal growth factor (EGF) stimulated intracellular Ca2+ mobilisation and PGE2 production in cultured amnion cells. The addition of a Ca2+ channel blocker (cobalt) or a Ca2+ chelator (EGTA) into the culture medium inhibited intracellular Ca2+ mobilisation and PGE2 production induced by EGF. The analysis of cell cycles showed that EGF induced the initiation of DNA synthesis and that the addition of cobalt or EGTA into the culture medium inhibited EGF-induced DNA synthesis. The addition of a cyclo-oxygenase inhibitor (indomethacin) inhibited PGE2 production and DNA synthesis induced by EGF without the effect on intracellular Ca2+ mobilisation. Moreover, the inhibitory effect of indomethacin on EGF-induced DNA synthesis was attenuated by the addition of exogenous PGE2 or PGF2 alpha.CONCLUSIONS:
These data suggest that EGF induces an increase in intracellular Ca2+ levels and the rate of prostaglandin production which leads to proliferation of human amnion cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dinoprostona
/
Cálcio
/
Fator de Crescimento Epidérmico
/
Âmnio
Tipo de estudo:
Observational_studies
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Br J Obstet Gynaecol
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Japão