ELISA for in vivo assessment of nonenzymatically glycated platelet glutathione peroxidase.
Clin Biochem
; 27(4): 293-8, 1994 Aug.
Article
em En
| MEDLINE
| ID: mdl-8001291
ABSTRACT
Using a combination of boronate affinity chromatography and ELISA methodology, a simple procedure was devised to selectively determine the in vivo glycated state of the platelet glutathione peroxidase (GSH-Px) from normal and diabetic subjects. The mean total GSH-Px levels in the normal (n = 14) and diabetic (n = 18) platelets were 1167 +/- 97 and 1007 +/- 73 ng/mg protein, respectively. The mean percentage glycated GSH-Px in the normal and diabetic platelets were 5.79 +/- 0.72% and 11.68 +/- 0.95%, respectively. When the percentage glycated GSH-Px was compared with the fructosamine values, a correlation coefficient of 0.71 was obtained. This indicates that the glycation status of platelet GSH-Px can be utilized as a sensitive, short-term index of plasma glucose levels.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Diabetes Mellitus Tipo 1
/
Glutationa Peroxidase
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Clin Biochem
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Canadá