Direct detection of Vibrio cholerae in stool samples.
J Clin Microbiol
; 32(5): 1246-8, 1994 May.
Article
em En
| MEDLINE
| ID: mdl-8051251
ABSTRACT
A direct method to detect Vibrio cholerae in stool samples was developed by using a PCR procedure that did not require a DNA purification step. Dilution (1/100) of stool samples prevented inhibition of the reaction by contaminants, and two consecutive PCRs, the second one with a nested primer, achieved the desired sensitivity. Comparison of the results obtained from stool swab samples processed by the two-step PCR and by an enzyme-linked immunosorbent assay using GM1 as the capture molecule showed that the former is more sensitive and gave positive results even when V. cholerae was not culturable or dead.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vibrio cholerae
/
Cólera
/
Reação em Cadeia da Polimerase
/
Fezes
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
País/Região como assunto:
America do sul
/
Argentina
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Argentina