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[Contribution of gene amplification in the biological diagnosis of toxoplasmosis]. / Apport de l'amplification génique au diagnostic biologique de la toxoplasmose.
Cazenave, J; Broussin, B; Verin, P; Tirard, V; Cheyrou, A; Begueret, J.
Afiliação
  • Cazenave J; Laboratoire de Biologie moléculaire, LABM, Bordeaux.
Presse Med ; 23(12): 573-5, 1994 Mar 26.
Article em Fr | MEDLINE | ID: mdl-8066060
Biological diagnosis of toxoplasmosis is generally based on indirect arguments (serology). In cases of immaturity or of immune depression, however, evidence of the parasite has to be obtained. This involves time-consuming or relatively insensitive culture techniques. Molecular biology, and more particularly the polymerase chain reaction gene amplification technique, makes it possible to identify an extremely small quantity of parasites in a complex biological fluid in a few hours. We summarized our experience with an original technique using toxoplasma ribosomal DNA as the target. Its use in the amniotic fluid provides a distinct improvement in antenatal diagnosis and is fast becoming the technique of reference. Difficult cases have been solved by its application in ophthalmology. Finally, in immunodepressed patients, especially in cases of acquired immunodeficiency syndrome, assessment is currently ongoing. The results obtained so far, especially in the analysis of the cerebral spinal fluid, are encouraging.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxoplasmose / Toxoplasmose Congênita / Toxoplasmose Ocular / Reação em Cadeia da Polimerase / Toxoplasmose Cerebral Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Adult / Humans / Newborn Idioma: Fr Revista: Presse Med Ano de publicação: 1994 Tipo de documento: Article País de publicação: França
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxoplasmose / Toxoplasmose Congênita / Toxoplasmose Ocular / Reação em Cadeia da Polimerase / Toxoplasmose Cerebral Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Adult / Humans / Newborn Idioma: Fr Revista: Presse Med Ano de publicação: 1994 Tipo de documento: Article País de publicação: França